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Image Search Results
Journal: Molecular Medicine Reports
Article Title: SNAIL transcription factor increases the motility and invasive capacity of prostate cancer cells
doi: 10.3892/mmr.2015.4585
Figure Lengend Snippet: Fold changes in the mRNA expression levels of SNAIL1 in LNCaP and PC3 cells 24 h post-transduction. The colors correspond to wild type (white), null control (vertical lines), Snail (black), shRNA control (horizontal lines) and shRNA Snail (grey) vectors. (A and B) mRNA expression levels of SNAIL1 increased only in the cells transduced with the SNAIL vector. (C and D) mRNA expression levels of SNAIL1 decreased only in the cells transduced with the vector encoding three shRNAs against the coding sequence region of SNAIL1. The data are presented as the fold change in mRNA expression levels of SNAIL1 following normalization to GAPDH and to the untransduced cells. *** P<0.001. shRNA, short hairpin RNA.
Article Snippet: In the present study, the LNCaP PCa cell line (cat. no. CRL-1740; American Type Culture Collection, Manassas, VA, USA) and
Techniques: Expressing, Transduction, Control, shRNA, Plasmid Preparation, Sequencing
Journal: Molecular Medicine Reports
Article Title: SNAIL transcription factor increases the motility and invasive capacity of prostate cancer cells
doi: 10.3892/mmr.2015.4585
Figure Lengend Snippet: Relative protein expression levels in the LNCaP and PC3 cells. Western blotting was performed to determine the relative protein expression levels of SNAIL1, E-cadherin and vimentin in the (A and B) LNCaP and (C and D) PC3 cells. In both cell lines the overexpression of SNAIL1 upregulated the expression levels of vimentin and downregulated the expression levels of E-cadherin. The cells with SNAIL1 silencing also exhibited upregulated expression levels of E-cadherin. The protein expression levels were normalized to α-tubulin. * P<0.05 and *** P<0.001. shRNA, short hairpin RNA.
Article Snippet: In the present study, the LNCaP PCa cell line (cat. no. CRL-1740; American Type Culture Collection, Manassas, VA, USA) and
Techniques: Expressing, Western Blot, Over Expression, shRNA
Journal: Molecular Medicine Reports
Article Title: SNAIL transcription factor increases the motility and invasive capacity of prostate cancer cells
doi: 10.3892/mmr.2015.4585
Figure Lengend Snippet: Survival percentage of LNCaP and PC3 cells overexpressing SNAIL1 or with SNAIL1 silencing. The overexpression of SNAIL1 caused a significant decrease in the survival of the (A) LNCap and (B) PC3 cells. Similarly silencing of SNAIL1 decreased the survival of the (C) LNCaP and (D) PC3 cells. Data were normalized to the untransduced cells. * P<0.05 and *** P<0.001 compared with the corresponding control (null or shRNA control). Values are expressed as the mean ± standard deviation. shRNA, short hairpin RNA.
Article Snippet: In the present study, the LNCaP PCa cell line (cat. no. CRL-1740; American Type Culture Collection, Manassas, VA, USA) and
Techniques: Over Expression, Control, shRNA, Standard Deviation
Journal: Molecular Medicine Reports
Article Title: SNAIL transcription factor increases the motility and invasive capacity of prostate cancer cells
doi: 10.3892/mmr.2015.4585
Figure Lengend Snippet: Percentages of LNCaP and PC3 cells positive for Ki-67. Significant decreases in cell proliferation were observed in the (A and B) LNCap and (C and D) PC3 cell lines following overexpression and silencing of SNAIL1. Data were normalized to the untransduced cells. (a) Wild type, (b) null, (c) Snail, (d) shRNA control and (e) shRNA Snail. ** P<0.01 and *** P<0.001 compared with the corresponding control (null or shRNA control). Values are expressed as the mean ± standard deviation. shRNA, short hairpin RNA.
Article Snippet: In the present study, the LNCaP PCa cell line (cat. no. CRL-1740; American Type Culture Collection, Manassas, VA, USA) and
Techniques: Over Expression, shRNA, Control, Standard Deviation
Journal: Molecular Medicine Reports
Article Title: SNAIL transcription factor increases the motility and invasive capacity of prostate cancer cells
doi: 10.3892/mmr.2015.4585
Figure Lengend Snippet: Changes in apoptosis in the LNCaP and PC3 cells at 24 h. The (A) LNCAP and (B) PC3 cell lines with SNAIL1 overexpression exhibited decreased levels of apoptosis, however, the (C) LNCAP and (D) PC3 cells with SNAIL1 silencing exhibited significantly increased levels of apoptosis. Data were normalized to the untransduced cells. *** P<0.001 compared with the corresponding control (null or shRNA control). Values are expressed as the mean ± standard deviation. shRNA, short hairpin RNA.
Article Snippet: In the present study, the LNCaP PCa cell line (cat. no. CRL-1740; American Type Culture Collection, Manassas, VA, USA) and
Techniques: Over Expression, Control, shRNA, Standard Deviation
Journal: Molecular Medicine Reports
Article Title: SNAIL transcription factor increases the motility and invasive capacity of prostate cancer cells
doi: 10.3892/mmr.2015.4585
Figure Lengend Snippet: Changes in migration of the LNCaP and PC3 cells at 24 h. The (A) LNCAP and (B) PC3 cell lines with SNAIL1 overexpression exhibited increased levels of migration, however (C) LNCAP and (D) PC3 cells with SNAIL1 silencing exhibited significant decreases in the levels of migration. Data were normalized to the untransduced cells. ** P<0,01 and *** P<0.001 compared with the corresponding control (null or shRNA control). Values are expressed as the mean ± standard deviation. shRNA, short hairpin RNA.
Article Snippet: In the present study, the LNCaP PCa cell line (cat. no. CRL-1740; American Type Culture Collection, Manassas, VA, USA) and
Techniques: Migration, Over Expression, Control, shRNA, Standard Deviation
Journal: Molecular Medicine Reports
Article Title: SNAIL transcription factor increases the motility and invasive capacity of prostate cancer cells
doi: 10.3892/mmr.2015.4585
Figure Lengend Snippet: Changes in invasion capacity of the LNCaP and PC3 cells at 24 h. The (A) LNCAP and (B) PC3 cell lines with SNAIL1 overexpression exhibited increased invasion capacity, however the (C) LNCAP and (D) PC3 cells with SNAIL1 silencing exhibited significantly decreased invasion capacity. Data were normalized to the untransduced cells *** P<0.001 compared with the corresponding control (null or shRNA control). Values are expressed as the mean ± standard deviation. shRNA, short hairpin RNA.
Article Snippet: In the present study, the LNCaP PCa cell line (cat. no. CRL-1740; American Type Culture Collection, Manassas, VA, USA) and
Techniques: Over Expression, Control, shRNA, Standard Deviation